Phologies and are immuno-positive for several neural markers. (A-C) Representative images > 자유게시판

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Phologies and are immuno-positive for several neural markers. (A-C) Representative images of Ni day 7 cells, fixed and stained for (A) HuC/D and Synaptophysin (Syn), (B) neural nuclei (NeuN) and Map2, or (C) Brn3a and Map2. Nuclei are stained with DAPI. (D) TuJ1 and Synaptophysin staining reveals the presence of growth cones (arrows) and presumptive synaptic boutons (star) in late-passage GG3.1 cultures on Ni day 7, indicating functional maturity. Bar indicates 50 m.Koehler et al. BMC Neuroscience 2011, 12:82 http://www.biomedcentral.com/1471-2202/12/Page 6 ofFigure 3 The effects of continual passaging on cellular morphology, colony shape and EB formation. (A) > 95 of colonies displayed spontaneous differentiation and loss of GFP expression in peripherally located cells of low-passage GG3.1 and miPS-25 cell clusters. Serial passaging results in morphological stability and uniform GFP expression in > 85 of cell colonies in miPS-25 cultures. (B) EB diameter increased after multiple passages (iPSC LP represents cells at P20-30) PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/15501003 compared to early-passage cultures (iPSC EP represents cells at P7-9). ESC-derived EB diameter also differs significantly from EP-derived EB (n = 3 for each group). Scale bars indicate 100 m. Values are mean ?SD. *P Capecitabine by CD24 cell surface expression. Histograms are representative results of 3 separate experiments are shown. Values are mean ?SD for 2-3 independent samples. *P < 0.05.iPSC derived neurons exhibit an improved functional profile after extended passagingTo evaluate the functiona.